ANTIBODY SERVICES

Monoclonal Antibody Development Service

BHAT BIO-TECH provides customized hybridoma development service to meet the customer needs. We will develop monoclonal antibodies to a wide range of antigens including peptides. All the animal experiments are carried out in our state-of-the art facility approved by CPCSEA, Govt. of India.

The monoclonal antibody development service is divided into four phases:

• Phase I: Immunization and evaluation of antibody titer
• Phase II: Fusion, plating and screening
• Phase III: Subcloning by limiting dilution, expansion and cryopreservation of 3 selected clones
• Phase IV: Amplification- production of 50 ml of culture supernatant containing monoclonal antibody.

Sample requirements:

• 5 mg of pure protein or 25-30 mg of peptide

Deliverables:

• Phase I:
• ELISA data for antibody titration
• Phase II:
• ELISA data for screening of hybridoma
• Culture supernatant from 5 of the best hybridoma containing wells.
• Phase III:
• ELISA data for screening antibody secreting sub-clones
• Culture supernatant from 3 of monoclones
• Isotyping results of 3 of the monoclonal antibody
• Phase IV:
• 50 ml of culture supernatant containing monoclonal antibody
• Frozen hybridoma cells
• Final project report

Additional Hybridoma related services:

• Additional sub-cloning, expansion and screening of parental clones
• Cryopreservation of up to 20 clones per month
• Monoclonal antibody production in cell culture flask- 50 to 100 ml culture supernatant
• Isotyping of monoclonal antibodies

Polyclonal Antibody Production Service in Rabbit, Chicken and Mice

At BHAT BIO-TECH, we develop custom polyclonal antibodies of highest quality with fast and reliable delivery times. The antibody screening will be done by direct ELISA and Western blotting.

Sample requirements:

• 5 mg of immunogen

Deliverables:

• Rabbit:
• 2ml of pre immunized rabbit serum
• 25ml to 30ml of immunized rabbit serum
• Chicken:
• Polyclonal IgY antibody.
• Mice:
• Pre immunized and immunized mice serum.

Antibody Purification Services:

BHAT BIO-TECH provides a wide range of options for the purification of monoclonal and polyclonal antibodies. These include relatively common methods such as protein A and protein G affinity as well as protein L and antigen affinity chromatography.

Sample requirements:

• Serum sample
• If antigen affinity chromatography is required 5 mg of antigen to prepare purification column

Deliverables:

• Purified antibody
• Project report

Antibody Labeling Services

BHAT BIO-TECH offers a wide range of antibody labeling services to meet the requirements of the researchers. Antibody conjugated to enzymes, biotin or FITC are widely used in a variety of immunotechniques as a detection reagent. We provide the following conjugation services

• Conjugation to HRP
• Conjugation to ALP
• Conjugation to FITC

Colloidal Gold Labeling Service

At BHAT BIO-TECH we have a strong expertise in rapid diagnostic kit development using colloidal gold conjugate. We offer conjugation of colloidal gold to a variety of proteins. We can conjugate proteins to colloidal gold of varying size such as 10nm, 15nm, 20nm, 40nm and 60nm depending on the customer requirements.

Features:

No aggregation

OD 540 is 10 or above

Uniform size

Wide range of particle size