PROTEIN EXPRESSION AND PURIFICATION SERVICES

At BHAT BIO-TECH, protein expression specialists work closely with clients to clone and express full-length recombinant proteins from full-length cDNAs, EST sequence information and genomic sequences, providing service and expertise at all stages of protein expression and purification. Protein expression and purification service package include sub-cloning into appropriate expression vector, sequence confirmation by gene sequencing, plasmid maxi preparation, optimization of protein expression, expression confirmation by SDS-PAGE and Western blotting, and purification of expressed protein to near homogeneity (>90% pure).

Features:

•   Protein expression in-Bacteria and Mammalian cells are available
•   Protein purification to near homogeneity (purity >90%)
•   Expression systems with 6X His tag, GST tag, GFP tag and without any tag are available
•   Large scale production of recombinant proteins  upon request
•   Purified proteins with Endotoxin level < 0.01EU/µg of protein is available on request

Deliverables:

• All clones and sub-clones generated
• Sequence data of the final clone
• Purified recombinant protein
• Project report
• Quality assurance certificate