DIFFERENT TYPES OF HIGH-QUALITY
SERVICES
DEPARTMENT
ANTIBODY SERVICES
Monoclonal Antibody Development Service
BHAT BIO-TECH provides customized hybridoma development service to meet the customer needs. We will develop monoclonal antibodies to a wide range of antigens including peptides. All the animal experiments are carried out in our state-of-the art facility approved by CPCSEA, Govt. of India.
The monoclonal antibody development service is divided into four phases:
- Phase I: Immunization and evaluation of antibody titer
- Phase II: Fusion, plating and screening
- Phase III: Subcloning by limiting dilution, expansion and cryopreservation of 3 selected clones
- Phase IV: Amplification- production of 50 ml of culture supernatant containing monoclonal antibody.
Sample requirements:
- 5 mg of pure protein or 25-30 mg of peptide
Deliverables:
- Phase I:
- ELISA data for antibody titration
- Phase II:
- ELISA data for screening of hybridoma
- Culture supernatant from 5 of the best hybridoma containing wells.
- Phase III:
- ELISA data for screening antibody secreting sub-clones
- Culture supernatant from 3 of monoclones
- Isotyping results of 3 of the monoclonal antibody
- Phase IV:
- 50 ml of culture supernatant containing monoclonal antibody
- Frozen hybridoma cells
- Final project report
| Cat. No. | Service |
| BBCS-20114 | Monoclonal antibody development service |
| BBCS-20114A | Phase I |
| BBCS-20114B | Phase II |
| BBCS-20114C | Phase III |
| BBCS-20114D | Phase IV |
Additional Hybridoma related services:
- Additional sub-cloning, expansion and screening of parental clones
- Cryopreservation of up to 20 clones per month
- Monoclonal antibody production in cell culture flask- 50 to 100 ml culture supernatant
- Isotyping of monoclonal antibodies
| Cat. No. | Service |
| BBCS-20116 | Monoclonal antibody production in cell culture flask |
| BBCS-20117 | Isotyping of monoclonal antibody |
Polyclonal Antibody Production Service in Rabbit, Chicken and Mice
At BHAT BIO-TECH, we develop custom polyclonal antibodies of highest quality with fast and reliable delivery times. The antibody screening will be done by direct ELISA and Western blotting.
Sample requirements:
- 5 mg of immunogen
Deliverables:
- Rabbit:
- 2ml of pre immunized rabbit serum
- 25ml to 30ml of immunized rabbit serum
- Chicken:
- Polyclonal IgY antibody.
- Mice:
- Pre immunized and immunized mice serum.
| Cat. No. | Service |
| BBCS-20118 | Polyclonal antibody production service in Rabbit |
| BBCS-20142 | Polyclonal antibody production service in Chicken |
| BBCS-20143 | Polyclonal antibody production service in Mice |
Antibody Purification Services:
BHAT BIO-TECH provides a wide range of options for the purification of monoclonal and polyclonal antibodies. These include relatively common methods such as protein A and protein G affinity as well as protein L and antigen affinity chromatography.
Sample requirements:
- Serum sample
- If antigen affinity chromatography is required 5 mg of antigen to prepare purification column
Deliverables:
- Purified antibody
- Project report
| Cat. No. | Service |
| BBCS-20119 | Purification of antibody by Protein A or protein G affinity chromatography |
| BBCS-20120 | Purification of antibody by Protein L affinity chromatography |
| BBCS-20121 | Purification of antibody by antigen affinity chromatography |
Antibody Labeling Services
BHAT BIO-TECH offers a wide range of antibody labeling services to meet the requirements of the researchers. Antibody conjugated to enzymes, biotin or FITC are widely used in a variety of immunotechniques as a detection reagent. We provide the following conjugation services
- Conjugation to HRP
- Conjugation to ALP
- Conjugation to FITC
| Cat. No. | Service |
| BBCS-20122 | Antibody- HRP conjugation |
| BBCS-20123 | Antibody- ALP conjugation |
| BBCS-20125 | Antibody- FITC conjugation |
Colloidal Gold Labeling Service
At BHAT BIO-TECH we have a strong expertise in rapid diagnostic kit development using colloidal gold conjugate. We offer conjugation of colloidal gold to a variety of proteins. We can conjugate proteins to colloidal gold of varying size such as 10nm, 15nm, 20nm, 40nm and 60nm depending on the customer requirements.
Features:
| Cat. No. | Service |
| BBCS-20129 | Colloidal gold labeling service |
IMMUNOASSAY DEVELOPMENT SERVICES
Our immunoassay development services are project-based and designed for diagnostics and drug discovery applications. Our scientists and project managers will discuss with each client the project design, cost estimates, and delivery specifications.
We offer three basic categories of services:
- Immunoassay development service for diagnostic markers
- Immunoassay development service for drug compound discovery
- Immunoassay development service for therapeutic antibody detection.
The immunoassay kit development is based on following methods
- ELISA assays
- Lateral flow assays
- Flow through assays
- Dipstick assays
In general our services include the following
- Antigen or immunogen preparation
- Antibody production, purification and modification
- Cell line generation
- Immunoassay development
- Reagent preparation
- Assay kit manufacturing
Deliverables:
- Deliverables vary depending on the types of services requested and the requirements of the assays.
- In general, we deliver cell lines, antibodies and conjugates, assay protocols, assay reagents, assay kits, experiment records, and specific data derived from a given project.
| Cat. No. | Service |
| BBCS-20141 | Immunoassay development services |
MICROBIAL BARCODING SERVICE
Microbial identification based on the gene sequencing has become the important tool for microbiologists. We offer following microbial identification services based on DNA sequencing. Identification up to species level and sometimes strain level is possible by this method.
- Bacterial identification service:
- It is based on PCR amplification of 1500bp 16S rRNA gene sequence. It involves amplification of 16S rRNA gene and sequencing of the PCR product.
- Actinomycetes Identification Service:
- It is based on PCR amplification of Actinomycetes species specific 750bp 16S rRNA gene sequence.
- Fungal Identification Service:
- It is based on PCR amplification of 650bp ITS region of the rRNA gene sequence.
- Yeast Identification Service
- It is based on PCR amplification of 500bp ITS region of the rRNA gene sequence
Features:
- Fast turnaround time
- Delivery time- 1 to 2 weeks
Deliverables:
- Detailed project report
- Gel image of PCR result
- Sequencing report
- Nucleotide sequence alignment within 10 most similar neighbors
- Phylogenetic tree displaying evolutionary relationship of 10 nearest neighbors
| Cat. No. | Service |
| BBCS-20130 | Bacterial identification service |
| BBCS-20131 | Actinomycetes identification service |
| BBCS-20132 | Fungal identification service |
| BBCS-20133 | Yeast identification service |
PROTEIN EXPRESSION AND PURIFICATION SERVICES
At BHAT BIO-TECH, protein expression specialists work closely with clients to clone and express full-length recombinant proteins from full-length cDNAs, EST sequence information and genomic sequences, providing service and expertise at all stages of protein expression and purification. Protein expression and purification service package include sub-cloning into appropriate expression vector, sequence confirmation by gene sequencing, plasmid maxi preparation, optimization of protein expression, expression confirmation by SDS-PAGE and Western blotting, and purification of expressed protein to near homogeneity (>90% pure).
Features:
- Protein expression in-Bacteria and Mammalian cells are available
- Protein purification to near homogeneity (purity >90%)
- Expression systems with 6X His tag, GST tag, GFP tag and without any tag are available
- Large scale production of recombinant proteins upon request
- Purified proteins with Endotoxin level < 0.01EU/µg of protein is available on request
Deliverables:
- All clones and sub-clones generated
- Sequence data of the final clone
- Purified recombinant protein
- Project report
- Quality assurance certificate
| Cat. No. | Service |
| BBCS-20110 | Protein expression in Bacteria |
WE ENSURE SAFE DIAGNOSES THERAPIES
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- Bone Slices
- Corticosterone EIA
- Bone Slices
- Corticosterone EIA
DNA VACCINES
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- Bone Slices
- Corticosterone EIA
- Dentine Discs
- Bone Slices
- Corticosterone EIA
- Dentine Discs
